The Columbus Lab
at the University of Virginia
Collaborations
Werner LM, Alcott A, Mohlin F, Ray JC, Belcher Dufrisne M, Smirnov A, Columbus L, Blom AM, Criss AK.
Endothelial alpha globin is a nitrite reductase.
Keller TCS 4th, Lechauve C, Keller AS, Broseghini-Filho GB, Butcher JT, Askew Page HR, Islam A, Tan ZY, DeLalio LJ, Brooks S, Sharma P, Hong K, Xu W, Padilha AS, Ruddiman CA, Best AK, Macal E, Kim-Shapiro DB, Christ G, Yan Z, Cortese-Krott MM, Ricart K, Patel R, Bender TP, Sonkusare SK, Weiss MJ, Ackerman H, Columbus L, Isakson BE.
Site-to-site cross-talk in OST-B glycosylation of hCEACAM1-IgV.
Conformational dynamics of the membrane enzyme LspA upon antibiotic and substrate binding.
Human CEACAM1 N-domain dimerization is independent from glycan modifications.
Variable Expression of Opa Proteins by Neisseria gonorrhoeae Influences Bacterial Association and Phagocytic Killing by Human Neutrophils.
The role of globins in cardiovascular physiology.
Imaging flow cytometry analysis of CEACAM binding to Opa-expressing Neisseria gonorrhoeae.
Werner LM*, Palmer A*, Smirnov A, Belcher-Dufrisne M, Columbus L, Criss AK
Cytometry: Part A. 97:1081-1089. (2020). PMC8062897
CEACAM targeted liposome delivery
Kuhn J, Smirnov A, Criss AK, Columbus L
Molecular Pharmaceutics. 16:2354 - 2363 (2019). PMC6740330
Heterocellular contact can dictate arterial function.
Shu XH, Ruddiman CA, Keller TCS, Keller AS, Yang Y, Good ME, Columbus L, Best AK, and Isakson BE.
Circulation Research. 124: 1473 – 1481 (2019). PMC6540980
The fluidity of phosphocholine and maltoside micelles and the effect of CHAPS.
Kieber M, Ono T, Oliver RC, Nyenhuis, SB, Ashtari M, Tieleman DP, Columbus L
Biophysical Journal. 116:1682 – 1691 (2019). PMC6506624
Refinement of highly flexible protein structures using simulation-guided spectroscopy.
Hays JM, Kieber MK, Li JZ, Han JI, Columbus L, Kasson PM.
Angnewandte Chemistry. 57:17110 – 17114 (2018). PMC6424112
Low q bicelles are mixed micelles.
Caldwell T*, Baoukina S*, Brock A, Oliver RC, Glover KJ, Tieleman DP, Columbus L
Journal of Physical Chemistry Letters. 9:4469 – 4473 (2018). PMC6353637
A novel conformation of the polypeptide-binding pocket supports an active substrate release from Hsp70s.
Yang J, Zong Y, Su J, Li H, Zhu H, Columbus L, Zhou L, and Liu Q.
Nature Communications 8:1201 – 1214 (2017). PMC5662698
Neisserial Opa protein – CEACAM interactions: competition for receptors as a means for bacterial invasion and pathogenesis.
Martin J, Ball L, Solomom T, Criss A, Columbus L.
Biochemistry 55: 4286 – 4294 (2016). PMC4980159
Modulating vascular hemodynamics with an alpha globin mimetic peptide (HbαX)
Keller TC, Butcher JT, Marziano C, Martin JN, Rogers S, Broseghini-Filho GB, Cabot M, Sgu X, Ning B, Best AK, Padilha AS, Purdy M, Yeager M, Peirce SM, Hu S, Doctor A, Barrett E, Le TH, Columbus L, Isakson BE.
Hypertension 68:1494 – 1503 (2016). PMC5159279
Endothelial nitric oxide synthase in the microcirculation.
Shu X*, Keller TC*, Begandt D, Butcher JT, Biwer L, Keller AS, Columbus L, Isakson BE.
Cellular and Molecular Life Sciences. 72:4561 – 4575 (2015). PMC4628887
Opa+ Neisseria gonorrhoeae has reduced survival in human neutrophils via Src family kinase-mediated bacterial trafficking into mature phagolysosomes.
Johnson MB, Ball LM, Daily KP, Martin JN, Columbus L, and Criss AK.
Cellular Microbiology. 17:648 – 665 (2015). PMC4402142
Cottrell Scholars Collaborative New Faculty Workshop: Professional Development for New Chemistry Faculty.
Baker LA, Chakraverty D, Columbus L, Feig AL, Jenks WS, Pilarz M, Stains M, Waterman R, and Wesemann J.
The Journal of Chemical Education. 91: 1874-1881 (2014).
Hemoglobin α/eNOS coupling in endothelium is required for nitric oxide scavenging during vasoconstriction
Straub AC, Mutchler S, Billaud M, Mykhaylo A, Palmer L, Le TH, Somlyo AV, Columbus L, Isakson BE.
Arteriosclerosis, Thrombosis, and Vascular Biology. 34:2594 – 2600 (2014). PMC4239174
The Structure of the Neisserial outer membrane protein Opa60: Loop flexibility essential to receptor recognition and bacterial engulfment.
Fox DA, Larsson P, Lo RH, Kroncke BM, Kasson P, Columbus L.
Journal of the American Chemical Society. 136:9938 – 9946 (2014). PMC4105060